Pcr products were detected on a 1 . 5 % agerose gel , stained by ethidium bromide . the bands containing the amplified fragments were visualized under uv illumination 5经漠化已锭染色的琼脂糖凝胶电泳后，紫外检测仪下观察扩增结果。
2.
A final period of extension was carried out for 9 min and final holding at 4 . the pcr products were resolved after electrophoresis in 2 % agarose gel with ethidium bromide . the pcr products were visualized while the gel was exposed to ultraviolet light 扩增产物在2琼脂糖凝胶上电泳，用pcrmarker作分子量标准，紫外灯下观察，最适pcr反应条件为扩增出最强的sry产物带而无非特异性扩增带出现时的反应条件。
3.
The acrystalliferous and plasmid - free derivatives of bacillus thuringiensis were screened with ethidium bromide treatment , elevating growth temperature from 42 to 44 then to 46 by degrees , and treating it with 0 . 05 % sodium docecyl sulphate ( sds ) as the plasmid - curing agent at 46 分别用溴化乙锭诱变处理、逐级升温培养以及用sds处理等三种方法对苏云金芽胞杆菌无晶体( cry ~ - )和无质粒突变株进行了筛选研究。
4.
The probe detection and pcr amplification are contemporary , without ethidium bromide staining and gel running and any post - pcr manipulations , so that the contamination risks are reduced . those two methods were used to detect successfully the primus necrotic ringspot virus from sample delivered by beijing airport quarantine bureau . the amplified fragment was cloned into the vector of pmd18 - t then sequenced 我们利用建立的杂交诱捕们pcr elisa和队f pcr对北京机场检疫局送检的樱桃样品检出了pnrsv ，同时把451hp的pcr产物克隆到ppo18 t载体上，通过序列测定，同源世最高的是genebank中pnrsv资料序号gi15750502gb山57046
5.
There is no acrystalliferous derivative was screened after treating 4 . 5 g / ml ethidium bromide , but after elevating growth temperature of ybt - 1463 and other 8 bacillus thuringiensis parental strains to 42 , 9 acrystalliferous derivative were obtained . a series of partially plasmid - cured derivatives were further obtained from 3 acrystalliferous derivatives originated from wild - type strain ybt - 1463 by elevating temperature to 44 用限量培养基和42培养9株野生菌株，结果筛选到9株cry ~ -突变株；继续升温至44来培养其中的三个cry ~ -突变株，得到内生质粒被进一步消除的突变株。
